Warning triangle appears during sample QC

Once a sample is assayed and is under review at the QC step, you may see an orange warning triangle, as in the image below. The orange triangle is to alert you to potential issues to review or resolve, but does not indicate a failure within the software or your data.

The table below provides the possible reasons for the warning triangle and related options available to you at the QC step.

Why do I see an orange triangle? Description Options/Actions Available Notes
You selected a sample status (for example: status= ‘Accept’) that is different than Ovation’s predicted status (for example: ‘Repeat’) based on that sample’s number of rejected assays Ovation automatically makes a sample status prediction based on the number of accepted, ignored, and rejected assays for that sample. The prediction depends on a threshold number (n), which is defined by your lab and based on your tests’ validation and SOPs. Given this threshold (n) and the number of actual rejected assays (R): If R = 0, then “Accept”If R < n,  then  “Repeat”If R > or = n, then “Reject”

If the user-selected status is different from the predicted status, Ovation shows the warning triangle to draw your attention to the sample.

You may choose to ignore the warning and continue with your selected sample status, or you may choose the recommended status based on the number of rejected assays. Please contact your customer success manager to update the threshold for rejected samples R in your configuration if Ovation does not have the threshold for your tests, or any time that R is changed in your SOP.
The sample’s assay data is outside of your designated QC parameters You may have QC parameters that have been configured for your assays and the sample data falls outside of these values. The sample status will also be auto-selected as ‘Reject’ or ‘Repeat’ in this case. You may choose to manually override the Reject/Repeat sample status by selecting ‘Accept’ from the drop-down. Please contact your customer success manager to setup or update your QC specifications.

Note: an unsigned requisition does not have a QC warning triangle. Instead, it shows a warning in the workflow saying the “Req Form not verified. Reports won’t be generated unless req form is verified.”  

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